CO2 extraction process of extracting ergosterol from Ganoderma lucidum

Ergosterol CO2 Extraction Processes
Ergosterol CO2 Extraction Processes

CO2 Extraction method of ergosterol from Ganoderma lucidum


The production of Ganoderma lucidum mycelium by deep fermentation is characterized by time-saving, labor-saving, environmental protection and easy control.

Supercritical CO2 fluid extraction is based on the relationship between the solubility of supercritical fluid and its density, that is, the influence of pressure and temperature on the solubility of supercritical fluid.

In the supercritical state, the supercritical fluid is contacted with the substance to be separated, so that it can selectively extract the components of polarity, boiling point and molecular weight successively, which has the characteristics of time-saving, labor-saving, environmental protection and good separation effect.

Based on the deep fermentation of Ganoderma lucidum fungus, the content of ergosterol in Ganoderma lucidum mycelium was extracted by CO2 supercritical, and the extraction conditions were optimized.

CO2 extraction

After deep fermentation of Ganoderma lucidum fungus for 120 hours, the mycelium and fermentation liquid are separated by centrifugation, the mycelium is taken, dried to constant weight in a constant temperature oven at 60 ℃, cooled to normal temperature in the dryer, weighed, mashed, sieved, 100 g of experimental sample is taken and put into the reaction kettle, CO2 gas is opened, at the same time, the temperature of extraction kettle, separation kettle I and separation kettle II is adjusted to the set temperature, and then pressurized to the set pressure.

Adjust the flow of CO2, make the supercritical CO2 fluid carry out the extraction cycle under the set pressure and temperature.

When the CO2 extraction time reaches the test set, receive the extraction liquid from the separation kettle I and the outlet of the separation kettle, combine the extraction liquid of the separation kettle I and the separation kettle II, and fix the volume to 500ml, then obtain the supercritical CO2 extraction liquid sample of Ganoderma lucidum fungus deep fermentation bacteria, for standby.


CO2 Extraction Machine Buyer’s Guide

As you can imagine, not all supercritical CO2 extractors are made the same.
For more than 30 years, we have customized many CO2 extraction machines (Tabletop CO2 Extractor) for many companies and university laboratories (1 lb CO2 extractor). Because each customer’s CO2 extraction process requirements are different, there are almost no identical machines.

How many liters of CO2 extraction vessel are needed?

The first factor that determines the size of the CO2 extraction vessel is the daily working hours of the plant, which determines the daily extraction batch. The vessel size of the CO2 extractor depends on the biomass to be processed per day.

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How many separators are needed?

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Waxes separation and Light oil separation in 1st separator;
Light oil separation in 2nd separator;
To trap lightest and volatile compounds in 3rd separator.
It is important to choose the right vessel size that will meet your current needs as well as future demand.

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How many pressure is best for your CO2 extraction process requirements?

The choice of CO2 extraction pressure is a changing factor. If the extraction pressure is too high, the useless substances in the plant will be extracted, such as wax and chlorophyll. If the extraction pressure is too low, the extraction time will be increased.

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How can the minimum operating costs be achieved?

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Further Reading: CBD Oil CO2 Extraction Machine

CO2 extraction process
CO2 extraction process

how to get an effective cO2 extraction process of ergosterol

In the process of extracting ergosterol from Ganoderma lucidum mycelium by supercritical CO2, single factor experiments show that the extraction pressure, extraction temperature and CO2 flow rate have different effects on the extraction amount of ergosterol.

L934 orthogonal experiment optimizes the extraction conditions. The results show that the extraction pressure and extraction temperature have significant effects, while the CO2 flow rate has no significant effects.

The appropriate combination of the three factors A2b3c3, i.e. extraction pressure 20 MPa, extraction temperature 50 ℃, CO2 flow rate 2.5 L / min, the extraction amount of ergosterol in this combination is 1.1362 mg / g, which is higher than that in the orthogonal combination.

Supercritical CO2 extraction of Ganoderma lucidum mycelium
Supercritical CO2 extraction of Ganoderma lucidum mycelium

Ganoderma lucidum

Ganoderma lucidum belongs to the basidiomycetes, the porous fungi, and the noble fungi with high medicinal value.

It has the functions of anti-inflammatory, diuretic, stomach invigorating, preventing arteriosclerosis, anti-tumor, anti-aging and improving immunity.


The research shows that there are many functional substances in Ganoderma lucidum, such as polysaccharides, triterpenoids, Ganoderma acid, ergosterol and so on.

The content of ergosterol in Ganoderma lucidum is high, It can reach more than 0.2%, the content of ergosterol in Ganoderma lucidum can reach UV irradiation.

Ergosterol can be converted into VD2 precursor,it is the main raw material for the production of progesterone, hydrocortisone, VD2, etc., with a large market demand.

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